The Doudna Lab

Exploring molecular mechanisms of RNA-mediated gene regulation

Collecting X-ray diffraction data at the Advanced Light Source Ancestral cGAS homologs reveal evolution of innate immune signaling Operating the ITC in our lab in Stanley Hall Macromolecular crystallography facilitated by robotics at UC Berkeley Analyzing RNA samples using gel electrophoresis in the lab

Recent publications

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes.

East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA.
NMol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.

CRISPR-Cas9 Structures and Mechanisms.

Jiang F, Doudna JA.
Annu Rev Biophys. 2017 Mar 30. doi: 10.1146/annurev-biophys-062215-010822. [Epub ahead of print]

Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery.

Gaj T, Staahl BT, Rodrigues GM, Limsirichai P, Ekman FK, Doudna JA, Schaffer DV.
Nucleic Acids Res. 2017 Mar 2. doi: 10.1093/nar/gkx154. [Epub ahead of print]

Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain.

Lintner NG, McClure KF, Petersen D, Londregan AT, Piotrowski DW, Wei L, Xiao J, Bolt M, Loria PM, Maguire B, Geoghegan KF, Huang A, Rolph T, Liras S, Doudna JA, Dullea RG, Cate JH.
PLoS Biol. 2017 Mar 21;15(3):e2001882. doi: 10.1371/journal.pbio.2001882. eCollection 2017.

RNA-based recognition and targeting: sowing the seeds of specificity.

Gorski SA, Vogel J, Doudna JA.
Nat Rev Mol Cell Biol. 2017 Feb 15. doi: 10.1038/nrm.2016.174. [Epub ahead of print]

News

New research and a paper by Jennifer, Alex, Dudu, Gavin and Mitch featured on UC Berkeley News


Check out the Research Highlight covering Jennifer and Brett's recent Nature Biotechnology paper. Cas9 cuts to the quick in the cortex. It is now live on Nature Lab Animal. Congratulations!


Mitch heads out to start his lab at the University of Rochester Medical Center. Congratulations and Best Wishes, Mitch!


Congratulations and Best Wishes to Romain Rouet who has finished is Postdoctoral work in the Doudna Lab and is now a Postdoctoral Fellow at the Garvan Institute of Medical Research in Sydney. Best Wishes, Romain!