40S-eIF3-eIF4G Cryo-EM

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Mapping of the eIF4G binding site on eIF3 and model of 40S complex with eIF3, eIF1, mRNA, and eIF4G.

1. Superposition of eIF4G density from difference mapping on the eIF3-IRES.
2. Model based on Fig. 4A and Fig. 3. The mRNA entry and exit sites are indicated.
3. Model of mRNA loading; e1 indicates eIF1 position. The A, P, and E sites are indicated. mRNA is depicted as a red line.

Reference:

• Siridechadilok, B., Fraser, C.S., Hall, R.J., Doudna, J.A. and Nogales, E. (2005) Structural roles for human translation factor eIF3 in initiation of protein synthesis.Science 310, 1513-1515. (216KB .pdf) (Supplemental material: 712KB .pdf) (Structure)

© 2005 The American Association for the Advancement of Science