Exploring molecular mechanisms of RNA-mediated gene regulation
Operating the ITC in our lab in Stanley Hall
Macromolecular crystallography facilitated by robotics at UC Berkeley
Collecting X-ray diffraction data at the Advanced Light Source
Analyzing RNA samples using gel electrophoresis in the lab
In bacteria, CRISPR systems preserve invading genetic material and incorporate it into surveillance complexes to achieve adaptive immunity. Cryo-EM structure of the CASCADE complex solved in collaboration with the Nogales Lab.
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Eukaryotes use a system known as RNA interference, or RNAi, to process host and viral nucleic acid into small interfering RNA (siRNA) or micro-RNA (miRNA) complexes, which then control gene expression. Work in the Doudna lab focuses on miRNA processing.
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The eukaryotic translation machinery is a critical control point for the induction of gene expression. Viruses by necessity hijack the translational equipment. Pictured is the HCV IRES which bypasses normal regulatory mechanisms to promote viral gene expression.
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