The Doudna Lab

Exploring molecular mechanisms of RNA-mediated gene regulation

Collecting X-ray diffraction data at the Advanced Light Source Ancestral cGAS homologs reveal evolution of innate immune signaling Operating the ITC in our lab in Stanley Hall Macromolecular crystallography facilitated by robotics at UC Berkeley Analyzing RNA samples using gel electrophoresis in the lab

Recent publications

ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing.

Chen X, Shen Y, Draper W, Buenrostro JD, Litzenburger U, Cho SW, Satpathy AT, Carter AC, Ghosh RP, East-Seletsky A, Doudna JA, Greenleaf WJ, Liphardt JT, Chang HY.
Nat Methods. 2016 Oct 17. doi: 10.1038/nmeth.4031. [Epub ahead of print]

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.

Hultquist JF, Schumann K, Woo JM, Manganaro L, McGregor MJ, Doudna J, Simon V, Krogan NJ, Marson A.
Cell Rep. 2016 Oct 25;17(5):1438-1452. doi: 10.1016/j.celrep.2016.09.080.

Insights into HIV-1 proviral transcription from the structure and dynamics of the Tat:AFF4:P-TEFb:TAR complex.

Schulze-Gahmen U, Echeverria I, Stjepanovic G, Bai Y, Lu H, Schneidman-Duhovny D, Doudna JA, Zhou Q, Sali A, Hurley JH.
Elife.2016 Oct 12;5. pii: e15910. doi: 10.7554/eLife.15910. [Epub ahead of print]

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.

East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA.
Nature. 2016 Sep 26. doi: 10.1038/nature19802. [Epub ahead of print]

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9.

Singh D, Sternberg SH, Fei J, Doudna JA, Ha T.
Nat Commun. 2016 Sep 14;7:12778. doi: 10.1038/ncomms12778.

Applications of CRISPR technologies in research and beyond.

Barrangou R, Doudna JA.
Nat Biotechnol. 2016 Sep 8:933-941. doi: 10.1038/nbt.3659. [Epub ahead of print]


Best wishes to Ireen who heads back to Germany to complete her studies at the Technical University of Darmstadt.

Welcome visiting scholars: Michael Schmitz and Eelco Meerdink come to work in the Doudna Lab!

Welcome new postdocs! April Pawluk, Chun-Hao Huang, Gavin Knott, Junjie Liu and Kyle Watters join the Doudna Lab!

David leaves to be a Research Fellow at University of Texas, Austin. Congratulations!

Jennifer Doudna is awarded the 2016 Tang Prize in Biopharmaeutical Science

Philip leaves for his new faculty position at Harvard, congrats Philip!

James leaves for his new postdoc position at UCSF, congrats James!