Exploring molecular mechanisms of RNA-mediated gene regulation
In bacteria, CRISPR systems preserve invading genetic material and incorporate it into surveillance complexes to achieve adaptive immunity. Crystal structures of diverse Cas9 proteins reveal RNA-mediated conformational activation.
Eukaryotes use a system known as RNA interference, or RNAi, to process host and viral nucleic acid into small interfering RNA (siRNA) or micro-RNA (miRNA) complexes, which then control gene expression. Work in the Doudna lab focuses on miRNA processing.
The eukaryotic translation machinery is a critical control point for the induction of gene expression. Viruses by necessity hijack the translational equipment. Pictured is the HCV IRES which bypasses normal regulatory mechanisms to promote viral gene expression.
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science 2016 Jan 14. pii: aad8282.
Wright AV, Nuñez JK, Doudna JA
Cell 2016 Jan 14;164(1-2):29-44.
Floor SN, Doudna JA
eLife 2016 Jan 6;5. pii: e10921
Nature 2015 Dec 24;528(7583):469-71
Doudna JA, Gersbach CA
Genome Biol 2015 Dec 23;16(1):292
Floor SN, Barkovich KJ, Condon KJ, Shokat KM, Doudna JA
Protein Sci 2015 Dec 9;