The Doudna Lab

Exploring molecular mechanisms of RNA-mediated gene regulation

Collecting X-ray diffraction data at the Advanced Light Source Ancestral cGAS homologs reveal evolution of innate immune signaling Operating the ITC in our lab in Stanley Hall Macromolecular crystallography facilitated by robotics at UC Berkeley Analyzing RNA samples using gel electrophoresis in the lab

Recent publications

GENOMES IN FOCUS: DEVELOPMENT AND APPLICATIONS OF CRISPR-CAS9 IMAGING TECHNOLOGIES.

Knight S, Tjian R, Doudna J.
Angew Chem Int Ed Engl. 2017 Oct 27. doi: 10.1002/anie.201709201. [Epub ahead of print]

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.

Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA.
Nature. 2017 Sep 20. doi: 10.1038/nature24268. [Epub ahead of print]

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.

Knott GJ, East-Seletsky A, Cofsky JC, Holton JM, Charles E, O'Connell MR, Doudna JA.
Nat Struct Mol Biol. 2017 Sep 11. doi: 10.1038/nsmb.3466. [Epub ahead of print]

A Broad-Spectrum Inhibitor of CRISPR-Cas9.

Harrington LB, Doxzen KW, Ma E, Liu JJ, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA.
Cell. 2017 Aug 23. pii: S0092-8674(17)30873-5. doi: 10.1016/j.cell.2017.07.037. [Epub ahead of print]

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9.

Dagdas YS, Chen JS, Sternberg SH, Doudna JA, Yildiz A.
Sci Adv. 2017 Aug 4;3(8):eaao0027. doi: 10.1126/sciadv.aao0027. eCollection 2017 Aug.

News

Congratulations to Postdoc Fuguo Jiang who was honored with the RNA Society/Scaringe Award, an award that was established to recognize the achievement of young scientists engaged in RNA research and to encourage them to pursue a career in the field of RNA. Fuguo was recognized for making great strides towards understanding the CRISPR-Cas9 genome editing complex, working alongside his colleagues in the Doudna lab. He has combined X-ray crystallographic data and single-particle cryo-electron microscopy approaches to describe the mechanism of DNA target recognition and key structural states of the Type II CRISPR-Cas9 family. These proteins are powerful tools from genome editing in a wide variety of organisms.


Congratulations to Postdoc Chun-Hao Huang who was selected as UNLEASH SDG talent and one of the winners selected to create solutions to UN Sustainable Development Goals.


Welcome to Ko-Chuan Lee who has joined the Doudna Lab as a visiting scholar. She will be with the lab until the summer of 2018. Welcome!


Doudna Lab alum, Rachel Haurwitz, made the "35 under 35" list in MIT Tech Review. Congratulations, Rachel!


Natalia Orlova and Brady Cress have joined the Doudna Lab as new postdocs. Welcome, Natalia and Brady!