The Doudna Lab

Exploring molecular mechanisms of RNA-mediated gene regulation

Collecting X-ray diffraction data at the Advanced Light Source Ancestral cGAS homologs reveal evolution of innate immune signaling Operating the ITC in our lab in Stanley Hall Macromolecular crystallography facilitated by robotics at UC Berkeley Analyzing RNA samples using gel electrophoresis in the lab

Recent publications

Disabling Cas9 by an anti-CRISPR DNA mimic.

Shin J, Jiang F, Liu JJ, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA.
Science Advances 2017 Jul 12;3(7):e1701620. doi: 10.1126/sciadv.1701620. eCollection 2017 Jul.

DNA recognition by an RNA-guided bacterial Argonaute.

Doxzen KW, Doudna JA.
PLoS One. 2017 May 17;12(5):e0177097. doi: 10.1371/journal.pone.0177097. eCollection 2017.

High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding.

Boyle EA, Andreasson JOL, Chircus LM, Sternberg SH, Wu MJ, Guegler CK, Doudna JA, Greenleaf WJ.
Proc Natl Acad Sci U S A. 2017 May 11. pii: 201700557. doi: 10.1073/pnas.1700557114. [Epub ahead of print]

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes.

East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA.
NMol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.

CRISPR-Cas9 Structures and Mechanisms.

Jiang F, Doudna JA.
Annu Rev Biophys. 2017 Mar 30. doi: 10.1146/annurev-biophys-062215-010822. [Epub ahead of print]

News

Natalia Orlova has joined the Doudna Lab as a postdoc. Welcome, Natalia!


Congratulations and Best wishes to Stephen Floor, Kevin Doxzen, Alex Seletsky, Spencer Knight and Eelco Meerdink who have recently left the Doudna Lab for new jobs or further studies!


Jennifer Doudna and Sam Sternberg's new book A Crack in Creation was released today, June 13!


New research and a paper by Jennifer, Alex, Dudu, Gavin and Mitch featured on UC Berkeley News


Check out the Research Highlight covering Jennifer and Brett's recent Nature Biotechnology paper. Cas9 cuts to the quick in the cortex. It is now live on Nature Lab Animal. Congratulations!